Integrated transcriptomic and proteomic analysis of microplasts derived from macrophage‐conditioned medium‐treated MCF‐7 breast cancer cells

Microplasts are large extracellular vesicles originating from migratory, invasive, and metastatic cancer cells. Here, to gain insight into the role of microplasts in progression, we performed a proteomic transcriptomic characterization isolated MCF-7 breast cells treated with macrophage-conditioned medium. These were found be viable, highly metabolically active, indicating that derived these not apoptotic bodies. Transcriptomic/proteomic analyses identified 10273 mRNAs 821 proteins microplasts. Interestingly, 377 microplast coded for corresponding proteins. Microplast mainly associated binding catalytic activities. showed enrichment involved transcription regulation processes such as cell–cell adhesion translation. Pathway analysis ribosomes carbon metabolism. results suggest close resemblance between parent cells, mRNA protein cargo relevant intercellular signaling.